Cell Behavior on Peptide-Immobilized Substrate with Cell Aggregation Inducing Property

Cell aggregates have been applied in various fields such as regenerative medicine and drug toxicity testing. We shown that H-(Lys-Pro)12-OH (KP24), a repeating sequence of lysine (Lys) proline (Pro), induces uniformly sized cell simply by its presence suspension. In this study, we considered peptide could be to three-dimensional culture substrate can induce uniform immobilizing it on the substrate. Therefore, mouse fibroblasts (L929) were seeded KP24-immobilized glass substrates behavior was observed. also human-derived cells, namely, human mesenchymal stem cells (hMSC), characterized their assemblies. Furthermore, KP24 chemically immobilized surface, which allowed us trace mechanism KP24–cell interaction. As analysis aggregation ability KP24, investigated whether interacts with surface without being incorporated into cell.